Materials and methods Strains and culture conditions Yeasts were maintained and grown either in YPD broth [1% yeast extract (Oxoid), 2% bacteriological peptone (Oxoid), 2% D-glucose], or in YNB broth [0.69% yeast nitrogen base without amino acids (Formedium), 2% D-glucose] supplemented as required with amino acids, uracil, 5-FOA (Melford), D-histidine (Sigma) or reduced GSH (Sigma)
reported the general paradigm that the elevated energy metabolism in ovarian cancer cell lines was directly associated with increased lactate levels ( et al
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Wang W, Lu Y, Xue Z, Li C, Wang C, Zhao X, et al
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