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Calcium assay The wild-type ghrelin receptor gene was sub-cloned in the pcDNA3.0 vector with an N-terminal HA signal peptide

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Time-lagged bolus injection of LINA after EMPA significantly increases USE in SD rats To study acute effects of LINA on USE under the presence or absence of SGLT2is, we performed acute injection experiments with EMPA alone, LINA alone, concurrent administration with EMPA and LINA, and time-lagged bolus injection of LINA an hour after EMPA into the SD rats

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