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See the full step-by-step protocol in the reconstitution section above

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Statistical analysis The sample size was calculated using G*Power 3.1.9.7 for Windows to detect an effect size of 0.4 with a power of 0.8 for adults and 0.9 for larvae and an alpha of 0.05 taking into account 5 experimental groups

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Nat Rev Neurol 12(8):455464 Global regional (2018) and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 19902017: a systematic analysis for the Global Burden of Disease Study 2017