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Biomarkers studied in more than one country included those related to the androgen receptor (AR), ribonuclease L (RNASEL), glutathione S-transferase enzymes T1, M1, and P1 (GSTM1, GSTT1, and GSTP1), cytochrome P450, 3-oxo-5-steroid 4-dehydrogenase 2 (SRD5A2), vitamin D receptor (VDR), and the non-coding region at 8q24.21

Twenty-four hours after seeding, cells were transfected with 15 g of plasmid DNA encoding different N-terminally HA-tagged S cytoplasmic tail mutants, and C-terminally FLAG-tagged M where specified, using PEI