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To analyze the effects on replacement of leucine-973 in the juxtamembrane region with phenylalanine, we generated preadipocytes in which both endogenous IR and IGF1R have been genetically inactivated to create double-knockout (DKO) cells using a Cre-lox recombination and then reconstituted these DKO cells with WT-IR or L973F-IR, both of which were FLAG tagged (Figure 1B)