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Growth media was replaced with stimulation buffer [phenol-free DMEM containing 0.1% (w/v) bovine serum albumin (BSA) and 1 mM 3-isobutyl-1-methylxanthine] and incubated for 1 h at 37 C in 5% CO 2

As we age, muscle mass becomes the primary driver of mobility and independence

Side Effects Comparison# Afamelanotide Side Effects (from clinical trials)# Melanotan-1 Side Effects (reported from unregulated use)# Regulatory and Legal Status# Key Differences Summary# Same molecule : Afamelanotide and melanotan-1 are pharmacologically identical -- [Nle4, D-Phe7]-alpha-MSH

Brauer GM, Stansbury JW

Vitamin E may assist in protecting heart tissue from oxidative damage