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Materials and methods An extensive literature search was carried out independently on online databases (MEDLINE via PubMed, ISI Web of Science, and Scopus), by four reviewers (RMR, EM, LC, and CV) independently of each other, to identify relevant studies published between January 2005 and June 2025

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Abbreviations MASLD: Metabolic dysfunction-associated steatotic liver disease MASH: Metabolic dysfunction-associated steatohepatitis WD: Western diet MC4R: Melanocortin 4 receptor KO: Knockout THR: Thyroid hormone receptor NAFLD: Nonalcoholic fatty liver disease NAS: Nonalcoholic fatty liver disease activity score AMLN: Amylin liver non-alcoholic steatohepatitis HFD: High fat diet DIO: Diet-induced obese GAN: Gubra-Amylin non-alcoholic steatohepatitis GLP-1: Glucagon-like peptide-1 MC: Methyl cellulose p.o.: Per os ALT: Alanine transaminase AST: Aspartate aminotransferase LDL-C: Low-density lipoprotein cholesterol TG: Triglyceride TC: Total cholesterol TIMP-1: Tissue inhibitor of metalloproteinase-1 ELISA: Enzyme-linked immunosorbent assay qRT-PCR: Quantitative real-time polymerase chain reaction Tnf: Tumor necrosis factor Il1b: Interleukin 1 beta Il6: Interleukin 6 Ccl2: C-C motif chemokine ligand 2 Col1a1: Collagen type 1 alpha 1 Col3a1: Collagen type 3 alpha 1 Spp1: Osteopontin Scd1: Stearoyl-Coenzyme A desaturase 1 Fabp4: Fatty acid binding protein 4 Mogat2: Monoacylglycerol O-acyltransferase 2 Gapdh: Glyceraldehyde-3-phosphate dehydrogenase HE: Hematoxylin and eosin ROI: Regions of interest RER: Respiratory exchange ratio LDLR: LDL receptor CDAA-HFD: Choline-deficient L-amino acid defined high fat diet References Bellentani, S

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J Clin Endocrinol Metab, 90(2), 611614