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This is done because the vial is a vacuum, and you need to add an equal amount of air to regulate the pressure

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doi:10.1016/j.jdin.2025.01.012

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Published laboratory investigations suggest it influences biological activity involving: Cellular migration Blood vessel formation Fibroblast activity Collagen organization Tissue remodeling Nitric oxide signaling Inflammatory response Scientists continue evaluating these biological pathways through controlled laboratory investigations designed to improve understanding of regenerative physiology