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sialic acid residues attached to proteins and lipids are major contributors to this negative charge

Liposomal glutathione encapsulates GSH within lipid bilayers, specifically designed to protect it from degradation and facilitate its absorption [ 1,2]

Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts