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LC-MS/MS proteomics analysis (isotopic labeling) Five hundred nanograms of digested sample from each treatment group were injected in technical triplicates

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Skin smooth 19

GHK-Cu stimulates collagen I, III, and elastin synthesis in fibroblasts Promotes decorin and glycosaminoglycan production for matrix integrity Attracts immune cells and promotes wound debridement and repair GHK-Cu may prevent oxidative stress in skin by regulating copper and modifying expression of numerous antioxidant genes2012Gene Expression Gene expression analysis reveals GHK-Cu upregulates antioxidant genes and downregulates pro-inflammatory genes, suggesting a protective role against oxidative stress and UV damage in skin

They also observed a significant positive correlation between estradiol and GPx