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Briefly, an aliquot of cell homogenate was incubated with the labeled substrate DEVD-pNA (acetyl-Asp-Glu-Val-Asp p-nitroaniline), which is cleaved by the caspase-3 enzyme and releases the chromophore pNA

What in-vitro work is still possible

For breeding, male VE-Cadherin-Cre ERT2 mice were crossed with female Igf1r f/f mice to generate VE-Cadherin-Cre ERT2 /Igf1r + / males, which were subsequently bred with Igf1r f/f females to establish the VE-Cadherin-Cre ERT2 /IGF1R homozygous floxed founder colony, following our previously described protocol [17, 45]

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Follow-Up and Monitoring We schedule follow-up appointments to track your body composition changes, assess progress, and adjust your protocol as needed