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This is a very important and possibly crucial biochemical mechanism, since reversible thiolation of proteins can modify their enzymatic activities (glycolytic enzymes, components of the respiratory chain, creatine kinase, carbonic anhydrase), ion and metabolite transport, gene transcription, signal transduction and cell death

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In that example, 25G is the gauge, and 1 inch is the length

Following the final wash, the cell pellet was resuspended in 1.5 ml of PBS containing 0.4% ( v / v ) NP40 alternative and flash-frozen in liquid nitrogen