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To induce DSBs, cells were treated with Z-(4)-Hydroxytamoxifen (Millipore Sigma, Cat#H7904, 1M) and Shield-1 (TaKaRa, Cat#632189, 1M) for a period of 4 hours in charcoal stripped FBS containing medium

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Thus, in mice, hepatocyte-specific overexpression of Dpp4 is associated with hepatic insulin resistance and liver steatosis (86), whereas knockdown of Dpp4 results in improved insulin sensitivity and reduced lipid accumulation in cultured hepatocytes (95)