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D+Q works by inhibiting pro-survival pathways (tyrosine kinases and Bcl-2 family) broadlythese pathways are also present in non-senescent cells, creating off-target effects

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The screening identified quinoliniums as a promising scaffold, and 5-Amino-1MQ emerged as one of the most active compounds with an IC 50 of approximately 1.2 M under the biochemical assay conditions used