Four MT concentrations (0, 50, 100, and 200 M) were used, and additional treatments with 100 M MT, 5 mM diphenyleneiodonium (DPI, an NADPH oxidase inhibitor suppressing H 2 O 2 production), or their combination were set to confirm the optimal MT concentration
2012) and that our ability to explore this experimentally is restricted by the limited metabolic capability and poor relevance to humans in vivo of many currently available in vitro toxicological models (Park et al
Proper laboratory handling involves using sterile techniques to prevent contamination during reconstitution, as these peptides are sensitive to environmental factors
Furthermore, in some tumours, the need for methyl groups for epigenetic regulation prevents activation of the trans-sulphuration pathway for cysteine synthesis, instead prioritising one-carbon metabolism
These observations, reflecting regulatory effects on the transcriptional control of transporters affecting brain UA, demonstrate tissue-specific effects on UA activity, which further question the validity of MR studies discussed above
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