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In fact, because of the then-dominating lock-and-key model of protein action and the associated central paradigm of structural biology stating that a unique 3D structure of a protein determines its function, all techniques for protein analysis were specifically designed for the analysis of the structural properties, conformational behavior, and thermodynamic stability of ordered proteins

2020;12(10):e11110

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Heparnized blood ( n =2) and bone marrow aspirates ( n =5) were used to isolate mononuclear cells by Ficoll density gradient centrifugation and cryopreserved using equal volumes of FBS and 15% dimethylsulphoxide dissolved in RPMI-1640 medium