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Lin PC, Lee MY, Wang WS, et al
The development of indeterminate symbiotic nodules (with prolonged meristem activity) involves processes such as root hair deformations, infection thread growth, the reactivation of cell division in the root inner cortex and pericycle followed by nodule primordium development, bacterial release, the differentiation of infected cells associated with repeated rounds of endoreduplication, and the differentiation of bacteria into nitrogen-fixing bacteroids ( The first rate-limiting step in the synthesis of both GSH and hGSH is the formation of -glutamylcysteine (-Glu-Cys) catalyzed by -glutamylcysteine synthetase (-ECS, encoded by GSH1 )
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Thus, the enrichment ratio for bt-miRNAbound mRNA was calculated as: (target gene PD/reference gene PD)/(target gene input)/(reference gene input)