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Relative photoluminescence intensity was then calculated from the ration between intensity at each time point to the initial intensity before irradiation

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Avoid common errors: Do not inject water directly onto the lyophilised cake (this creates excessive foaming) Do not shake the vial vigorously (this creates foam and potentially damages peptide) Do not overfill the vial with excessive bacteriostatic water (this may compromise sterility during reconstitution) Do not use the solution if it remains cloudy after 5 minutes (potential contamination) Reconstituted Solution Storage Temperature and Duration Once reconstituted, IGF-1 LR3 solutions are more susceptible to degradation: Optimal storage: 2-8C (refrigeration) in a light-protected container