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The NADH oxidation assay showed that the initial reaction velocity of 6-hydroxy-FAD-reconstituted hFSP1 was fast similar to that of native purified hFSP1, but decreased rapidly after 3 min, which maybe resulted from the poor stability of 6-hydroxy-FAD-reconstituted hFSP1, while the initial reaction velocity of FAD-reconstituted hFSP1 was obviously slower than that of 6-hydroxy-FAD-reconstituted hFSP1, but almost constant until the reaction was complete (Supplementary Fig

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