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The effect of metformin on aminotransferase levels, metabolic parameters and body mass index in nonalcoholic fatty liver disease patients: a metaanalysis

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The resulting mixtures were then washed and concentrated to approximately 20 g/l using Amicon 3 kDa molecular weight cutoff filters (Millipore, UFC5003BK) in protein buffer containing 30 mM Tris-HCl, 150 mM NaCl, 1 mM -mercaptoethanol, pH = 7.5

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