However, other methods have been developed recently that are reported to be more efficient, reaching levels of 70% oxidisation, by using increased quantities of potassium permanganate, and adding phosphoric acid combined with the sulphuric acid, instead of adding sodium nitrate
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Additionally, GSH plays a crucial role as a co-substrate for enzyme GPx, which is in charge of detoxifying the H 2 O 2 and lipid hydroperoxides, as well as direct neutralization of AFT (by forming an AFTGSH conjugation) ( are built up, which aggressively attack the phospholipid membrane, leading to MDA accumulation ( Accordingly, oxidative damage is prominently emphasized in our study, expounded by an evident drop in GSH levels and the activities of SOD and GPx in renal tissues