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The following day, assay media was prepared by supplementing specialized DMEM pH 7.4 (Agilent, 103575-100) with 10% dFBS, 1% penicillinstreptomycin, 25 mM glucose and 2 mM L-glutamine

Store lyophilized at 20 C

TrimRx's questionnaire took about ten minutes, and I heard back from a provider the next morning