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It also decreases the risk that differences in sample processing times are seen between freshly collected healthy controls and 2-day old patient samples could be attributable to differences seen between cohorts, as opposed to pathological differences, during biomarker developmentas has been hypothesised with the ATP Profile test [136]

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The in vivo passive cutaneous anaphylaxis (PCA) assay was used to measure IgG1a antibody responses, but ELISA methods have now been developed that have eliminated some of the variability seen with the PCA