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The supernatant was transferred to LCMS vials, and the pellet was lysed by 20 mM NaOH and protein levels were detected for normalization

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Methycobal Injection is commonly prescribed for individuals with Vitamin B12 deficiency, peripheral neuropathy, megaloblastic anemia caused by Vitamin B12 deficiency, and other conditions where Vitamin B12 replacement is clinically indicated

Future studies should also consider the impact of extra-neurological injury such as renal or hepatic impairment on the PK of 7,8-DHF