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gspD is an essential gene in M

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Fluorescence quantitation All images used for quantitation were acquired using the sequential scan mode on the Leica TCS SP8, under the same conditions across all samples

We also observed strong LAG-1::HA staining in nuclei of all somatic gonad cells, the DTC, and all sheath and spermathecal cells, as well as in polyploidy intestinal cells, in both the L4 and adult stage dissection preparations