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Safety studies have evaluated single doses up to 0.4 grams per kilogram of body weight (approximately 28 grams for a 70-kilogram adult) without serious adverse effects, though gastrointestinal symptoms represent the primary tolerability concern

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Supernatants were collected and the protein concentrations were determined using a BCA assay kit (Pierce/Thermo Scientific, Rockford, IL) for Western blot analysis and high performance liquid chromatography (HPLC) assay

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