Final treatment decisions are made by licensed clinicians based on individual medical history, clinical evaluation, and professional judgment
They're optimised for their conversion rate
Then, and only then, add strategically
Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency
However, the first week is typically more about the body adjusting to the medication rather than seeing significant changes on the scale