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Competent yeast cells were transformed with yeast-display plasmid pCTCON2 following the Frozen EZ yeast transformation II (Zymo Research, T2001) according to the manufacturers protocol

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AQ-QI and DEAQ-QI were dissolved in DMF, stored at -80C and protected from light to prevent possible degradation

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Furthermore, the rate of rebound weight gain following the cessation of GLP-1R agonists, as well as the implementation of future strategies to avoid this phenomenon, are other issues that need to be thoroughly addressed in future studies [65]