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For all IF stainings, wells were imaged with a 20 objective except BrdU staining (imaged using a 10 objective)

Our explainer on how tirzepatide influences incretin pathways and the overview at what tirzepatide (Mounjaro/Zepbound) is and how it works walk through the receptor pharmacology

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This may possibly suggest that the introduction of the peptide may have caused a proliferation of somatotroph cells, which are the natural cells with GH-producing potential in the pituitary gland