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The intestinal barrier is primarily maintained by tight junctions (TJs) between epithelial cells, which regulate intestinal permeability and prevent the invasion of pathogens and harmful substances into the submucosal layer

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After 48 h, the measurement was started by switching from DMEM medium to preheated glucose starvation minimal medium (140 mM NaCl, 5 mM KCl, 1 mM of MgCl 2 , 2 mM of CaCl 2 , 20 mM Hepes, pH 7.4 as adjusted with NaOH) with 10% FCS

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Usually, 2 to 3 mg of vitamin B 12 reserves are stored in the body primarily in the liver, and our daily requirement of vitamin B 12 is only about 2 to 3 g