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Reconstitution reference U100 units to draw for each common BPC-157 dose, by vial size, reconstituted with 2 mL of bacteriostatic water

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For each sample, 200 l of supernatant was added to 600 l of thiobarbituric acid (TBA) solution, reconstituted in glacial acetic acid, and then incubated at 95 C for 60 min in a dry-block thermostat (Bio TDB-100, Biosan, Latvia) and finally cooled immediately on ice for 10 min