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The other piece was used for biochemical analysis

Product Highlights & Quick Facts Product Type: Triple-Peptide Blend (Combination Growth Hormone Releasing Hormone & Analogues) Total Content: 12mg per vial (specific breakdown ratio [verify with brand/source]) Format: Lyophilized (freeze-dried) white powder or cake Purity Standard: Greater than or equal to 99% [verify with brand/source Certificate of Analysis] Primary Intended Use: In vitro laboratory research and comparative animal models Storage Requirements: Stable at room temperature during transit

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For LC-MS/MS analysis, 20 L of each sample was injected into Sciex Exion LC AC system coupled with SCIEX Triple TOF 5600 mass spectrometer (AB SCIEX, Canada)