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Schirmer RH, Adler H, Pickhardt M, Mandelkow E (2011) Lest we forget you-methylene blue

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After a 2D classification with a binned dataset in CRYOSPARC, a homogeneous subset of particles was re-extracted as the native pixel sampling and subjected to Bayesian particle polishing in RELION, followed by another further 2D classification in cryoSPARC

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