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Biotinylated goat anti-mouse IgG or goat anti-rabbit IgG (Vector Labs, BA-9200 1:200 and BA-1000 1:200, respectively) secondary antibodies were added in blocking buffer and incubated for 1 h at room temperature

Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial growth and extends reconstituted peptide shelf life from 48 hours (sterile water) to 28 days (refrigerated)

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Zhou, B., Honor, L

On day 28, there were significant improvements in SODA scores for all three groups in the pain, non-pain, and satisfaction categories, and by day 56 these improvements had become more pronounced