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The amount of lipid-peroxidation was determined using a TBARS Assay Kit (Cayman Chemical, Cat#10009055), per manufacturer instructions and absorbance was measured at a wavelength between 530540 nm using a plate reader

Multiple bioinformatic approaches revealed that high expression of five genes ( ZnT1 , 5-7 , and 9 ) was significantly correlated with better overall survival (OS), first progression survival (FPS), and post-progression survival (PPS), while upregulated ZnT2-4 , 8 , and 10 expressions was markedly associated with poor OS, FP, and PPS