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Fresh stock solutions of NADPH, GSH, GR and GSSCys or HEDS were prepared in assay buffer containing 0.1 M Tris/HCl, 1 mM EDTA, pH 8.0 before each experiment

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For example, a novel GSH formulation bypassing the gastrointestinal digestion through an oral absorption, gave positive results in raising GSH blood concentration in vitro and in vivo (31)

The ring tension was manually adjusted to 1.0 g and equilibrated for 30 min

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