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Microdialysis experiments required local alcohol administration, achieved by diluting alcohol in modified Ringers solution (140 mM NaCl, 1.2 mM CaCl, 3.0 mM KCl, and 1.0 mM MgCl, Sigma-Aldrich, Darmstadt, Germany) to 300 mM, corresponding to approximately 50-60 mM outside the probe in the NAc (81)
Managing Side Effects Start Low, Go Slow Starting with a low dose and gradually increasing it can help minimize side effects, particularly gastrointestinal ones
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