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Each TrxR1 and acTrxR1 enzyme variant (1 M) was incubated in phosphate buffer (100 mM potassium phosphate, 1 mM EDTA, pH 7.0) with increasing peroxide concentrations (0500 M H 2 O 2 (16,911-250ML-F, Sigma)) for 1 h in a total volume of 200 l at 37C

These findings suggest that NRF2 activation exerts protective effects against ferroptosis by reducing lipid accumulation and oxidative stress, thereby mitigating NAFLD progression

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