This creates a gentle current within the solution that allows the remaining powder to dissolve fully without causing any mechanical stress to the peptide molecules
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a , HT1080 OE GFP-GPX4 WT and HT1080 OE GFP-GPX4 R152H were treated with 4 M RSL3, 30 g/ml cycloheximide, and 100 M a-Tocopherol for 0, 2, 4, or 6 hours before Western Blot analysis of GPX4 and GADPH