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To promote in vivo recombination in yeast, the genes were PCR-amplified to introduce specific overhangs of 4055 bp to the linearized vector using iProof high-fidelity polymerase (Bio-Rad, 1725301) following the manufacturers specifications

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10.1080/14740338.2020.1772230 [DOI] [PubMed] [Google Scholar] 44.Aroda VR, Bain SC, Cariou B, Pileti M, Rose L, Axelsen M, et al

Powerful Anti-Inflammatory and Antioxidant Actions : Research shows that GHK-Cu reduces inflammatory cytokines such as IL-6 and TNF- while enhancing antioxidant enzyme expression, including superoxide dismutase and catalase

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