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Targeting LDHB sensitizes tumor cells to radiotherapy by enhancing mitochondria-associated ferroptosis It has been shown previously that exposure of cancer cells to ionizing radiation resulted in the induction of ferroptosis 29 , which was associated with morphological changes similar to those observed in this study after silencing LDHB (Fig

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Then, 15 g of protein was mixed with NativePAGE sample buffer (Invitrogen), Coomassie G-250 and protease inhibitor, loaded into the lanes of a 312% Bis-Tris NativePAGE gel (Invitrogen) and run according to the manufacturers instructions

Suitable pH adjusting agents or buffers include, but are not limited to acetate, bicarbonate, ammonium chloride, citrate, phosphate, pharmaceutically acceptable salts thereof and combinations or mixtures thereof