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Reduced airway surface pH impairs bacterial killing in the porcine cystic fibrosis lung

For quantitative real-time PCR (qPCR) assays, expression levels of rat 2A -adrenergic receptor ( Adra2a ) were detected using the QuantiTect SYBR Green PCR Kit (Qiagen) and SYBR Select Master Mix (Sigma-Aldrich) and quantified according to the 2 Ct method

The main signs and symptoms of Carnitine Deficiency Syndrome include low muscle tone and muscle weakness in the neck and jaw, shoulders and upper arms, hips and legs, and even in the heart