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IGF-1 availability is tightly regulated by the so-called insulin- like growth factor binding proteins (IGFBPs), which may act by increasing IGF-1 half-life, from minutes to hours (most commonly by forming a tertiary complex with Acid-Labile Subunit and IGFBP3), however blocking its binding to the insulin-like growth factor 1 receptor (IGF- 1R) [7]
RNA extraction for microarray and quantitative real-time RT-PCR (Q-PCR) The RNAs from different cell lines were isolated using TRIzol reagent (Invitrogen, #15596018), and then the cDNAs were reverse transcribed using the Super Script III Reverse Transcriptase system (Invitrogen, #18080051)
However, this outcome was reported in a subset of trials and showed less consistent statistical significance in sensitivity analyses, suggesting potential benefits in cardiac function and disease progression
Growth Hormone & IGF Research, 16 (2), 132-142