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Immunoprecipitation was then performed using the Catch and Release Immunoprecipitation Kit (EMD Millipore), according to the manufacturers instructions with 250 g of total protein from cells or 400 g from tissues, and 1 g of an anti-IGF-1R antibody (#9750, Cell Signaling)

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If you take time-sensitive medications like thyroid hormones or oral contraceptives, discuss timing adjustments with your prescriber

The benzyl alcohol preservative in bacteriostatic water slows microbial growth but does not stop peptide degradation