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There are several methods, but the best is the carbon dioxide method, which uses no chemicals

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(2020) Acta Crystallogr D Struct Biol 76 : 1222-1232 PubMed: 33263328 Search on PubMedSearch on PubMed Central DOI: PubMed Abstract: The growth of diffraction-quality crystals and experimental phasing remain two of the main bottlenecks in protein crystallography

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