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The concentration of complementary deoxyribonucleic acid (cDNA) after reverse transcription were also determined using NanoDrop Spectrophotometer

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Caspase-3 activity was markedly elevated, indicating enhanced apoptosis

Key Takeaways 5-Amino-1MQ inhibits nicotinamide N-methyltransferase (NNMT), increasing cellular NAD+ levels by preserving nicotinamide for NAD+ salvage pathways rather than methylation to N-methyl nicotinamide