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The "timing hypothesis" or "critical window" hypothesis, now supported by substantial mechanistic and clinical data, proposes that estrogen's cardiovascular protective effects are contingent on initiating therapy while vascular endothelium remains estrogen-responsive, generally within ten years of menopause onset or before age 60 [8]

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Compounds were formulated with 10% DMSO and 10% Tween-80, and vortexed to obtain a uniform mixture

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