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Determination of cell death by PI staining Cells were trypsinized, washed with PBS and suspended in 300 l Dulbeccos phosphate buffered saline (DPBS) supplemented with 0.2% BSA containing 0.3 g/ml propidium iodine (PI, Sigma-Aldrich)

ROS trigger a cascade of events through nuclear factors activation (NF-kappa B), which up-regulates gene expression of pro-inflammatory cytokines that mediate the immune responses causing inflammation

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Stable isotope D9-TMA and D9-TMAO (Cambridge Isotope Laboratories, Inc.) were added to the samples as the internal standard for the TMA and TMAO quantification, respectively

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