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doi:10.4088/PCC.08l00707

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For immunostainings cells were blocked for 30 min in blocking buffer (10% GS, 0.3% Triton-X-100, 20 mM HEPES pH 7.4), then incubated with anti-p62 antibody (1:200, BD Bioscience, 610832), washed three times for 10 min in PBS, incubated with AF568-labeled goat-anti-mouse antibody, washed again three times in PBS for 10 min, and finally mounted using Shandon TM Immu-Mount TM containing Hoechst33342

Compared to constructs without IGF-1 overexpression, IGF-1-overexpressing constructs significantly promoted osteochondral repair and reduced cartilage degeneration near the defect

In the context of "healthy aging," glutathione helps protect the mitochondriathe powerhouses of your cells