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the amount, route, and copper load are what determine biological effect and risk, and those remain uncharacterized in humans

PLoS ONE 11 , e0162590

Int J Mol Sci 26:2902

Hidaka, T., Fujii, K., Funahashi, I., Fukutomi, N., & Hosoe, K

This colourimetric assay is based on the ability of antioxidants to reduce the ferric tripyridyl triazine (Fe 3+ -TPTZ) complex to the ferrous form at a low pH